sFLT01 modulates invasion and metastasis in prostate cancer DU145 cells by inhibition of VEGF/GRP78/MMP2&9 axis

Abstract Background About 90% of cancer-related deaths are due to metastasis cancer cells, and angiogenesis is a critical step in this process. sFLT01 novel fusion protein dual-targeting agent that neutralizes both VEGF PlGF proangiogenic activities. GRP78 dual effect tumor growth could be activated under stimulation. The current study was designed investigate the inhibitory impact on VEGF/GRP78 axis. To point, construct synthesized, recombinant plasmid expressed eukaryotic host sFLT01-HisTag extracted analyzed. functional activity VEGF-enhanced tube formation HUVEC cells were examined. Eventually, growth, invasiveness, migration human prostate cell line, DU145, assessed. Real-time PCR evaluated level its downstream factors; matrix metallopeptidase proteins 2&9 (MMP2&9) along with tissue inhibitor metalloproteinase proteins1&2 (TIMP1&2) Results According data, showed modulatory proliferation, invasion, DU145 potential HUVECs angiogenesis. Real-Time analysis depicted significant downregulation GRP78, MMP2 MMP9 transcripts’ levels, subsequent elevation TIMP1 TIMP2 expression stimulation detected. Conclusion Overall, these data indicated invasiveness mediated through modulation VEGF/GRP78/MMP2&9 axis activation TIMPs.